1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer with stilbene was investigated by using phase-contrast and fluorescence microscopy, differential scanning calorimetry, and Fourier transform infrared and UV–visible absorption spectroscopy. The results indicate that the temperature of the gel–liquid-crystalline phase transition of the DPPC bilayer with cis-stilbene is much lower than that with trans isomer. Photoirradiation to trans-stilbene in the DPPC bilayer suppresses the pretransition (Lβ′ to Pβ′), while the gel–liquid-crystalline phase transition (Pβ′ to Lα) changes little because of the poor photoisomerization rate in both gel and liquid-crystalline phases. It is shown that the photoisomerization of incorporated stilbene and the phase behavior of the lipid bilayer depend on each other.
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