Sign in  |  Register
Cart

CSJ Journals

▾
  • -

2018, Vol.47, No.1

107-109

Chondrocyte Shapes and Detachment on a Thermoresponsive Poly(2-methoxyethyl acrylate) Analog for the Development of New Chondrocytes Subculture Substrate

Takashi Hoshiba ,*1,2,3Kazuhiro Sato ,4Naoki Kawazoe ,3Guoping Chen ,3 and Masaru Tanaka *1,5
1Frontier Center for Organic Materials, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan
2Innovative Flex Course for Frontier Organic Material Systems, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Japan
3Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
4Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan
5Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
https://doi.org/10.1246/cl.170889

Tools
PDF (907K)
Abstract

Chondrocyte dedifferentiation during subculture prevents cartilage tissue engineering. The dedifferentiation is generally caused by cell spreading and trypsin treatment. Thus, it is expected that chondrocytes maintain their specific functions when the cells are subcultured on a substrate that can suppress cell spreading and allow the cells to detach without trypsin treatment. Here, we showed that thermoresponsive poly(2-methoxyethyl acrylate) and analogous polymers can be used to suppress cell spreading and detachment without trypsin treatment.

figure

Chondrocyte dedifferentiation is led by cell spreading and trypsin treatment. Thus, it is expected that chondrocytes maintain their specific functions when the cells are subcultured on the substrate which can suppress cell spreading and allow the cells to detach without trypsin treatment. Thermoresponsive poly(2-methoxyethyl acrylate) analogous polymers can be used for suppressing chondrocyte spreading and detachment without trypsin treatment.

<< Previous Hydrogenolysis of Tetrahydrofurfuryl Alcohol to 1,5-Pentanediol over a Nickel-Yttrium Oxide Catalyst Containing Ruthenium Next >> Improved Synthesis of Fluocinolone Acetonide and Process Research of 6α,9α-Fluorination

Select Issues

▾
  • -

Search for Articles

Advanced

Saved Search

Please login to view your saved searches.

Top Accessed Articles

Most Cited

Related Articles

  1. Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms
  2. Grafting Poly(ethylene glycol) to a Glass Surface via a Photocleavable Linker for Light-induced Cell Micropatterning and Cell Proliferation Control
  3. Contribution of Fibroblasts Cultured on 3D Silica Nonwoven Fabrics to Cocultured Hepatocytes Function
  4. Simple Method to Fabricate an Biocompatible Antibacterial Surface on a Versatile Substrate through an Antiadhesion Approach
  5. Development of Positive Photoresist for Controlling Cell Culture Shape on Organic Substrates
MANAGE YOUR ALERTS
RECOMMEND TO LIBRARY