Sign in  |  Register
Cart

CSJ Journals

▾
  • -

2021, Vol.50, No.12

2011-2014

Protein Triad Comprising Genetically Fused Hemoglobin and Human Serum Albumins as an Artificial O2 Carrier Resistant to Haptoglobin Binding

Yoshitsugu Morita ,Yuki Shindo , and Teruyuki Komatsu *
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
https://doi.org/10.1246/cl.210549

Tools
PDF (1692K)
Abstract

We describe synthesis of a symmetric protein triad comprising a genetically fused recombinant hemoglobin(di-α/Cβ120) center and human serum albumin sides [rHb(di-α/Cβ120)–HSA2]. This trimer is not cleaved at low concentration. It shows adequate resistance to haptoglobin binding as an artificial O2 carrier used as a red blood cell (RBC) substitute. Also, O2 affinity of the trimer was modulated to the appropriate value, similarly to that of RBCs, by complexation of inositol hexaphosphate and a Kβ108 mutation into the Hb core.

figure

A genetically fused recombinant hemoglobin [rHb(di-α/Cβ120)] was coupled with two human serum albumins (HSAs), yielding a rHb(di-α/Cβ120)–HSA2 trimer as an artificial O2 carrier. The di-α fusion prevents cleavage of the Hb core and haptoglobin binding. O2 affinity can be tuned to the appropriate value by complexation of an inositol hexaphosphate and a Kβ108 mutation into the Hb core.

<< Previous Structure-preserving Solid-state Thermal Huisgen Cycloaddition Polymerization of a Self-assembled Triptycene-based AB3-type Monomer Next >> Formation of Self-organized Structures of Hybrid Amorphous Materials Based on Polyhedral Silsesquioxanes

Select Issues

▾
  • -

Search for Articles

Advanced

Saved Search

Please login to view your saved searches.

Top Accessed Articles

Most Cited

Related Articles

  1. Determination of Oxygen Binding Properties of the Individual Subunits of Intact Human Adult Hemoglobin
  2. Suppression of Methemoglobin Formation by Glutathione in a Concentrated Hemoglobin Solution and in a Hemoglobin-Vesicle
  3. Coordination Behavior of O2 and CO in a Solid Film Consisting of Hemoglobin and Maltose
  4. Polarographic Studies on Oxygen Transport by Human Hemoglobin in Buffer Solutions
  5. Selective Detection of Human Serum Albumin Using a Fused-Silica Capillary Modified with Anti-Human Serum Albumin
MANAGE YOUR ALERTS
RECOMMEND TO LIBRARY